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pe cy7 conjugated mab against cd3  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec pe cy7 conjugated mab against cd3
    CD4 + T cell infiltration in the peripheral blood (PB) and joint-derived samples (SF, SM)
    Pe Cy7 Conjugated Mab Against Cd3, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe cy7 conjugated mab against cd3/product/Miltenyi Biotec
    Average 99 stars, based on 5 article reviews
    pe cy7 conjugated mab against cd3 - by Bioz Stars, 2026-05
    99/100 stars

    Images

    1) Product Images from "Proinflammatory T cell polarization is already present in patients with early knee osteoarthritis"

    Article Title: Proinflammatory T cell polarization is already present in patients with early knee osteoarthritis

    Journal: Arthritis Research & Therapy

    doi: 10.1186/s13075-020-02410-w

    CD4 + T cell infiltration in the peripheral blood (PB) and joint-derived samples (SF, SM)
    Figure Legend Snippet: CD4 + T cell infiltration in the peripheral blood (PB) and joint-derived samples (SF, SM)

    Techniques Used: Cell Counting

    Flow cytometry analysis of CD4 + T cell surface marker expression. Representative flow cytometry histograms of CD4 + T cell surface marker expression are shown. In short, mononuclear cells were isolated from PB, SF, and SM by density gradient centrifugation. CD3 + MACS-isolated T cells from PB, SF, and SM were stained with monoclonal antibodies (mAb) for the following surface markers: CD4, CD25, CD127, CXCR3, CCR5, CCR3, CCR4, CD161, and CCR6. Before flow cytometric detection, 7-AAD was added to the cell suspensions to exclude cell debris and dead cells. The cutoff for all cell surface markers was defined based on fluorescence minus one (FMO) controls (shown as gray overlay population). mAb, monoclonal antibody; MACS, magnetic-activated cell sorting
    Figure Legend Snippet: Flow cytometry analysis of CD4 + T cell surface marker expression. Representative flow cytometry histograms of CD4 + T cell surface marker expression are shown. In short, mononuclear cells were isolated from PB, SF, and SM by density gradient centrifugation. CD3 + MACS-isolated T cells from PB, SF, and SM were stained with monoclonal antibodies (mAb) for the following surface markers: CD4, CD25, CD127, CXCR3, CCR5, CCR3, CCR4, CD161, and CCR6. Before flow cytometric detection, 7-AAD was added to the cell suspensions to exclude cell debris and dead cells. The cutoff for all cell surface markers was defined based on fluorescence minus one (FMO) controls (shown as gray overlay population). mAb, monoclonal antibody; MACS, magnetic-activated cell sorting

    Techniques Used: Flow Cytometry, Marker, Expressing, Isolation, Gradient Centrifugation, Staining, Bioprocessing, Fluorescence, FACS

    Cytokine secretion of activated CD3 + CD4 + CD8 − T cells in the peripheral blood (PB) and joint-derived samples (SF, SM)
    Figure Legend Snippet: Cytokine secretion of activated CD3 + CD4 + CD8 − T cells in the peripheral blood (PB) and joint-derived samples (SF, SM)

    Techniques Used:



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    Image Search Results


    CD4 + T cell infiltration in the peripheral blood (PB) and joint-derived samples (SF, SM)

    Journal: Arthritis Research & Therapy

    Article Title: Proinflammatory T cell polarization is already present in patients with early knee osteoarthritis

    doi: 10.1186/s13075-020-02410-w

    Figure Lengend Snippet: CD4 + T cell infiltration in the peripheral blood (PB) and joint-derived samples (SF, SM)

    Article Snippet: For surface marker expression, PE-Cy7-conjugated mAb against CD3 (clone SK7), APC-Cy7-conjugated mAb against CD4 (clone RPA-T4), and VioBlue-labeled mAb against CD8 (clone BW135/80, Miltenyi Biotec, Germany) were used, and staining was performed at 4 °C for 30 min.

    Techniques: Cell Counting

    Flow cytometry analysis of CD4 + T cell surface marker expression. Representative flow cytometry histograms of CD4 + T cell surface marker expression are shown. In short, mononuclear cells were isolated from PB, SF, and SM by density gradient centrifugation. CD3 + MACS-isolated T cells from PB, SF, and SM were stained with monoclonal antibodies (mAb) for the following surface markers: CD4, CD25, CD127, CXCR3, CCR5, CCR3, CCR4, CD161, and CCR6. Before flow cytometric detection, 7-AAD was added to the cell suspensions to exclude cell debris and dead cells. The cutoff for all cell surface markers was defined based on fluorescence minus one (FMO) controls (shown as gray overlay population). mAb, monoclonal antibody; MACS, magnetic-activated cell sorting

    Journal: Arthritis Research & Therapy

    Article Title: Proinflammatory T cell polarization is already present in patients with early knee osteoarthritis

    doi: 10.1186/s13075-020-02410-w

    Figure Lengend Snippet: Flow cytometry analysis of CD4 + T cell surface marker expression. Representative flow cytometry histograms of CD4 + T cell surface marker expression are shown. In short, mononuclear cells were isolated from PB, SF, and SM by density gradient centrifugation. CD3 + MACS-isolated T cells from PB, SF, and SM were stained with monoclonal antibodies (mAb) for the following surface markers: CD4, CD25, CD127, CXCR3, CCR5, CCR3, CCR4, CD161, and CCR6. Before flow cytometric detection, 7-AAD was added to the cell suspensions to exclude cell debris and dead cells. The cutoff for all cell surface markers was defined based on fluorescence minus one (FMO) controls (shown as gray overlay population). mAb, monoclonal antibody; MACS, magnetic-activated cell sorting

    Article Snippet: For surface marker expression, PE-Cy7-conjugated mAb against CD3 (clone SK7), APC-Cy7-conjugated mAb against CD4 (clone RPA-T4), and VioBlue-labeled mAb against CD8 (clone BW135/80, Miltenyi Biotec, Germany) were used, and staining was performed at 4 °C for 30 min.

    Techniques: Flow Cytometry, Marker, Expressing, Isolation, Gradient Centrifugation, Staining, Bioprocessing, Fluorescence, FACS

    Cytokine secretion of activated CD3 + CD4 + CD8 − T cells in the peripheral blood (PB) and joint-derived samples (SF, SM)

    Journal: Arthritis Research & Therapy

    Article Title: Proinflammatory T cell polarization is already present in patients with early knee osteoarthritis

    doi: 10.1186/s13075-020-02410-w

    Figure Lengend Snippet: Cytokine secretion of activated CD3 + CD4 + CD8 − T cells in the peripheral blood (PB) and joint-derived samples (SF, SM)

    Article Snippet: For surface marker expression, PE-Cy7-conjugated mAb against CD3 (clone SK7), APC-Cy7-conjugated mAb against CD4 (clone RPA-T4), and VioBlue-labeled mAb against CD8 (clone BW135/80, Miltenyi Biotec, Germany) were used, and staining was performed at 4 °C for 30 min.

    Techniques: